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Computer Program for Primer Design for Loop-Mediated Isothermal Amplification (LAMP)

Introduction. To date, numerous methods of nucleic acid amplification have been proposed, and each method has a number of advantages and disadvantages. One of the most popular methods is Loop-mediated isothermal AMPlification (LAMP). Unlike thermocyclic reactions, such as PCR (polymerase chain reaction), which require three temperature changes and expensive equipment, in LAMP, the entire reaction takes place at one and the same temperature and at the maximum rate possible. An important component of LAMP-amplification is primers (usually 20–25 nucleotides), which need to be matched to a specific part of the nucleotide sequence. It is known that DNA sequence contains four nucleotides: A — adenine and T — thymine, G — guanine and C — cytosine. There is a huge variety of permutations of these nucleotides, and it is practically impossible to analyze such a large amount of data manually. Therefore, there is a need to use modern computer technologies. More than 150 computer programs have been proposed for the design of PCR primers, while for LAMP-primers there are less than 10 of them, and each of them has a number of drawbacks, e.g., in terms of the length of the analyzed site. Therefore

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